She began her musical education by studying the violin. Having graduated from a state secondary music school in Białystok, she gained her M.A. in musicology from the Academy of Catholic Theology in Warsaw (1998). In 1998–2002 she attended the Postgraduate Course in Voice Production in the class of Jadwiga-Gałęska-Tritt at the Music Academy in Bydgoszcz, gaining an honours diploma.

In 1993–2004 she worked closely with the Choir of the Cardinal Stefan Wyszyński University (formerly the Academy of Catholic Theology) as a singer, soloist and vocal coach. In 2000–2004 she taught voice production at the Cardinal Stefan Wyszyński University and in 2004–2016 was on the faculty of the Music Academy in Bydgoszcz (postgraduate choral studies and voice technique). She also worked with the Warsaw University Academic Choir (2005–2016).   Since 2005 she has served as vocal coach of the ‘Musica Sacra’ Choir of the Warsaw-Praga Cathedral.

Joanna Łukaszewska has also developed a solo career, appearing in premieres of works by such contemporary Polish composers as Anna Ignatowicz, Marcin Łukaszewski, Łukasz Farcinkiewicz, Paweł Łukaszewski, Stanisław Moryto and Marian Sawa. She has participated in numerous festivals: ‘Gaude Mater’ International Festival of Sacred Music in Częstochowa (1997, 1998), 12th International Evenings of Organ and Chamber Music in Kłodzko (1998), 7th Organ Evenings in Legnica (1998), Interpretations of Choral Music at the Music Academy in Warsaw (1998), 13th Conversatorium of Organ Music in Legnica (1998), 6th ‘Lubuska Camerata’ International Summer Festival of Chamber Music (2003). Foreign tours have taken her to France (Rouen Cathedral), Germany and Italy (Rome, Milan Cathedral, ‘Music World’ International Choral Festival in Fivizzano in 1998).

Her discography comprises 10 CDs with solo parts including Moryto’s Lenten Psalms and Marian Sawa’s Stabat Mater. ‘Musica caelestis’ received the Fryderyk Award of the Polish recording industry in 2016 and ‘Musica Sacra Poloniae’ (DUX 0108) was nominated for the award in 1999 and received the Grand Prix at the 14th International Festival of Catholic Films and Multimedia in Niepokalanów (1999).

The CD ‘Ave Maria’, in which Joanna Łukaszewska sang alongside Małgorzata Walewska, received the Golden Disc and Platinum Disc titles in 2002. She has performed with Einbecker Kantorei, the Polish Radio Symphony Orchestra, the Polish Army Concert Orchestra, the Polish Army Artistic Ensemble and Lubulska Sinfonietta conducted by Ulrike Hastedt, Andrzej Banasiewicz, Grzegorz Mielimąka, Jan Łukaszewski, Bolesław Szulia and Kazimierz Szymonik. In 2003 she sang in a concert featuring works by Marian Sawa and in a series of performances of Paweł Łukaszewski’s Litany to the Martyrs of Międzyrzecz.

She is a member of the Phonographic Academy. She runs workshops and training sessions in collective and individual voice production across Poland, is a juror in numerous competitions and is working on her doctoral dissertation at the Fryderyk Chopin University of Music. In 2016 she joined the University’s faculty as a lecturer in voice production